Hi Yield® Mini Plasmid DNA Extraction Kit

The Hi Yield^® Plasmid Mini Kit was designed for both plasmid and cosmid DNA purification. This plasmid kit will purify plasmid DNA/cosmid DNA from 1-5 ml of cultured bacterial cells using an efficient plasmid miniprep system. The Hi Yield^® Plasmid Mini Kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the plasmid spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer, TE or water. Typical yields are 20-30 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 ml of cultured bacterial cells. This plasmid kit does not require DNA phenol extraction or alcohol precipitation and the entire procedure can be completed within 15 minutes.

Advantages

• Purify plasmid DNA or cosmid DNA within 15 minutes!
• High Yield: up to 30 mg of pure plasmid DNA or cosmid DNA
• Sample Volume: 1-5 ml of bacterial cells
• Spin Columns: glass fiber membrane optimized for plasmid DNA purification
• Storage: dry at room temperature (15-25 °C) for up to 1 year