Hi Yield® gSYNC DNA Extraction Kit

The Hi Yield^® gSYNC DNA Extraction Kit is a genomic DNA extraction kit optimized for genomic, mitochondrial and virus DNA purification from whole blood (fresh blood and frozen blood), serum, plasma, buffy coat, body fluids, cultured cells, tissue, rodent tails, ear punches, formalin-fixed paraffin-embedded tissue (FFPE), amniotic fluid, hair and insects in one convenient kit.

This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water.

The entire procedure can be completed in less than 20 minutes without phenol/chloroform extraction or alcohol precipitation.

Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.

Advantages

• Purify genomic DNA within 20 minutes!
• Variety of Samples: 50-200 µl of whole blood, plasma, serum, buffy coat, up to 25 mg of tissue, rodent tails, ear punches, insects, up to 1 x 10⁷ cultured cells, up to 25 mg of formalin-fixed-paraffin-embedded tissue, up to 15 ml of amniotic fluid, hair
• High Yield: up to 5 µg from whole blood
• High Quality DNA: A260/A280 = 1.8-2.0
• Spin Columns: glass fiber membrane optimized for genomic DNA extraction
• Storage: dry at room temperature (15-25°C) for up to 1 year

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• Probenvolumen:
  • 200 µl Blut
  • 25 mg tierisches Gewebe
  • 1 x 10⁷ Zellkultur
• Hohe Ausbeute:
  • Bis zu 5 µg aus Vollblut
  • 10-40 µg aus 20 mg Gewebe
• Spin column: Glas-Fiber Matrix - grüne Membran optimiert für genomische DNA Extraktion
• Bindekapazität: 50 µg
• Elutionsvolumen: 30-100 µl
• Aufreinigungszeit: 20 min